Antioxidant and Antimicrobial Properties of Cistus Ladanifer

Merve Deniz Köse, Buse Nur Tekin, Oguz Bayraktar, Emre Taylan Duman, Yücel Başpınar


Different parts of Cistus species are traditionally used in folk medicine as a remedy for several microbial disorders and infections. At the beginning of the study, crude extract of the Cistus leaves was obtained to determine its antioxidant and antimicrobial activities. Then volatile compounds were extracted using hydrodistillation and hexane. The Cistus oil was obtained by hydrodistillation of fresh material, using leaves in an altered Clevenger-type device. Aqueous extract of Cistus leaves was neutralized to precipitate gums. The yield of gum was determined as 1%. Resinoid was obtained after ethanol extraction of gum. Antibacterial activities of extract were determined using disc diffusion and micro-dilution assays against gram-positive and gram-negative bacteria. The extract of Cistus leaves tested and exhibited antibacterial activities by inhibiting one or more microorganisms. The tested plant extract was more active against gram-positive bacteria compared with gram-negative bacteria. Total phenol content of the extract was determined with Folin-ciocalteu method. Total phenolic content of Cistus extract was 520 ± 15 mg Gallic Acid Equivalent (GAE)/ g extract. The water soluble (ACW) and lipid soluble (ACL) antioxidant capacities of the extract were also determined. ACW and ACL antioxidant capacities of Cistus extract were found as 650±80 μg Ascorbic acid/ mg extract and 540 ±30 μg Trolox Equivalent/ mg extract, respectively. In this study, there is a clear relationship between the total antioxidant and phenol content analysis results and antibacterial activities. Hereby, bioactive natural compounds present in Cistus species can be used as natural raw material in some related industrial applications.


Cistus genus, Antibacterial activity, Antioxidant capacity, Labdanum

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. Lubbe, A., & Verpoorte, R. (2011). Cultivation of medicinal and aromatic plants for specialty industrial materials. Industrial Crops and Products, 34(1), 785-801.

. Rios, J. L., & Recio, M. C. (2005). Medicinal plants and antimicrobial activity. Journal of ethnopharmacology, 100(1), 80-84.

. Atlas, R. M., Brown, A. E., & Parks, L. C. (1995). Laboratory manual of experimental microbiology. Mosby.

. Cetin, H., & Yanikoglu, A. (2006). A study of the larvicidal activity of Origanum (Labiatae) species from southwest Turkey. Journal of Vector ecology, 31(1), 118-122.

. Vertuani, S., Braccioli, E., Buzzoni, V., & Manfredini, S. (2002). Antioxidant capacity of Adansonia digitata fruit pulp and leaves. Acta phytotherapeutica, 2(5), 2-7.

. Barros, L., Dueñas, M., Alves, C. T., Silva, S., Henriques, M., Santos-Buelga, C., & Ferreira, I. C. (2013). Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts. Industrial Crops and Products, 41, 41-45.

. Popov, I., & Lewin, G. (1999). Antioxidative homeostasis: characterization by means of chemiluminescent technique. Methods in enzymology, 300, 437-456.

. Singleton, V. L. (1999). Analysis of total phenols and other oxidation substrates and their antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzymol., 299, 152-178.

. Kuete, V., Metuno, R., Ngameni, B., Tsafack, A. M., Ngandeu, F., Fotso, G. W., & Beng, V. P. (2007). Antimicrobial activity of the methanolic extracts and compounds from Treculia obovoidea (Moraceae). Journal of ethnopharmacology, 112(3), 531-536.

.Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African journal of biotechnology, 5(11).

. Harrison, K., & Were, L. M. (2007). Effect of gamma irradiation on total phenolic content yield and antioxidant capacity of almond skin extracts. Food Chemistry, 102(3), 932-937.

Zakaria, Z., Sreenivasan, S., & Mohamad, M. (2007). Antimicrobial Activity of Piper ribesoides Root Extract Against Staphylococcus aureus. Journal of Applied Biological Sciences, 1(3).

.Maggi, F., Lucarini, D., Papa, F., Peron, G., & Dall'Acqua, S. (2016). Phytochemical analysis of the labdanum-poor Cistus creticus subsp. eriocephalus (Viv.) Greuter et Burdet growing in central Italy. Biochemical Systematics and Ecology, 66, 50-57.

.Morales-Soto, A., Oruna-Concha, M. J., Elmore, J. S., Barrajón-Catalán, E., Micol, V., Roldán, C., & Segura-Carretero, A. (2015). Volatile profile of Spanish Cistus plants as sources of antimicrobials for industrial applications. Industrial Crops and Products, 74, 425-433.

.Skorić, M., Todorović, S., Gligorijević, N., Janković, R., Živković, S., Ristić, M., & Radulović, S. (2012). Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp. creticus L. on human cancer cell lines. Industrial Crops and Products, 38, 153-159.

.Paolini, J., Falchi, A., Quilichini, Y., Desjobert, J. M., De Cian, M. C., Varesi, L., & Costa, J. (2009). Morphological, chemical and genetic differentiation of two subspecies of Cistus creticus L.(C. creticus subsp. eriocephalus and C. creticus subsp. corsicus). Phytochemistry, 70(9), 1146-1160.


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