The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line

Sedat Çetin

Abstract


The present study was planned to investigate the potential protective properties of lycopene, an antioxidant carotenoid, on NaF applied renal cell line. NRK-52E cells were cultured under standard in vitro conditions with regular passages. 10.000 NRK-52E cells were planted in each culture platelet. Cells were incubated for 24 hours at 37°C in an incubator with CO2. After the incubation, the medium formed on the cells was removed and the prepared NaF and lycopene solutions were added. At least 4 wells were used for each dose. Culture vessels were incubated at 37°C in a CO2 incubator for 6, 12, and 24 hours for transformation of non-soluble formazan crystals by MTT stain and the MTT assay was conducted. In conclusion, it was found that low lycopene concentrations reduced the toxic effect of NaF by 10-20%, while lycopene increased the toxic effect of NaF synergistically in the high concentration lycopene treated groups. Thus, it was concluded that administration of lycopene on NaF applied renal cell line exhibited different effects based on concentration and duration.

Keywords


NaF, Lycopene, Cell Culture, MTT, Kidney.

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References


. Bramley, P.M. (2000). Is lycopene benefcial to human health? Phytochemistry, 54(3), 233-236.

. Agarwal, A., Shen, H., Agarwal, S., & Rao, A.V. (2001). Lycopene content of tomato products: its stability, bio availability and in vivo antioxidan properties. J Med Food, 4(1), 9-15.

. Pruthi, R.S, Derksen, E., & Gaston, K.(2003). Cyclooxygenase-2 as a potential target in the prevention and treatment of genitourinary tumors, a review. J Urol, 169(6), 2352-2359.

.Mashima, R., Witting, P.K., & Stocker, R. (2001). Oxidants and antioxidants in atherosclerosis. Curr Opin Lipidol, 12(4), 411-418.

. Xu, H., Hu, L.S., Chang, M., Jing, L., Zhang, X.Y., & Li, G.S (2005) Proteomic analysis of kidney in fluoride-treated rat. Toxi Lett, 160 (1), 69–75.

. Chouhan, S., & Flora, S.J. (2008). Effects of fluoride on the tissue oxidative stress and apoptosis in rats: Biochemical assays supported by IR spectroscopy data. Toxicology, 254(1- 2), 61–67.

. Çetin, S. (2017). NaF Application in Renal and Osteoblast Cell Lines. The Effects of Certain Minerals on Apoptosis and DNA Damage. Yuzuncu Yıl University, Institute of Health Sciences, PhD Thesis.

. Matos, H.R., Capelozzi, V.L., Gomes, O.F., Mascio, P.D., & Medeiros, M.H. (2001). Lycopene inhibitis DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys, 396(2), 171-177.

. Li, W., Jiang, B., Cao, X., Xie, Y., & Huang, T. (2017). Protective effect of lycopene on fluoride-induced ameloblasts apoptosis and dental fluorosis through oxidative stress-mediated Caspase pathways.Chem Biol Interact, 261(5), 27-34.

. Mansour, H.H., & Tawfik, S.S. (2012). Efficacy of lycopene against fluoride toxicity in rats. Pharm Biol, 50(6), 707-711.


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