The effects of lycopen application on sodium fluoride (NaF) applied renal cell line

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sedat çetin

Abstract

The present study was planned to investigate the potential protective effect of lycopene, an antioxidant carotenoid, on NaF applied renal cell line. NRK-52E cells were cultured under standard in vitro conditions with regular passages. 10,000 cells were planted in each culture platelet well for NRK-52E cells. Cells were incubated for 24 hours at 37°C in an incubator that contained CO2. After the incubation, the medium on the cells was removed and the prepared NaF and lycopene solutions were added. At least 4 wells were used for each dose. Culture vessels were incubated at 37°C in a CO2 incubator for 6, 12, and 24 hours so that MTT stain could transform non-soluble formazan crystals and MTT assay was conducted.In conclusion, it was found that low lycopene doses reduced the toxic effect of NaF by 10-20%, whereas in the high dose lycopene treated groups, lycopene increased the toxic effect of NaF synergistically. As a result, it was concluded that administration of lycopene on NaF applied renal cell line exhibited different effects based on the dose and time.The present study was planned to investigate the potential protective effect of lycopene, an antioxidant carotenoid, on NaF applied renal cell line. NRK-52E cells were cultured under standard in vitro conditions with regular passages. 10,000 cells were planted in each culture platelet well for NRK-52E cells. Cells were incubated for 24 hours at 37°C in an incubator that contained CO2. After the incubation, the medium on the cells was removed and the prepared NaF and lycopene solutions were added. At least 4 wells were used for each dose. Culture vessels were incubated at 37°C in a CO2 incubator for 6, 12, and 24 hours so that MTT stain could transform non-soluble formazan crystals and MTT assay was conducted.In conclusion, it was found that low lycopene doses reduced the toxic effect of NaF by 10-20%, whereas in the high dose lycopene treated groups, lycopene increased the toxic effect of NaF synergistically. As a result, it was concluded that administration of lycopene on NaF applied renal cell line exhibited different effects based on the dose and time.

Article Details

Section
Sending to International Journal of Secondary Metabolite
Author Biography

sedat çetin, Yuzuncu Yil University van Turkey

veterinery medicine Biochemstry