In Vitro Evaluation of Antimicrobial and Antibiofilm Potentials of Essential Oil of Tanacetum argenteum (Lam.) Willd. subsp. canum (K.Koch) Grierson var. canum

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Nazime Mercan Doğan Arzu Cansaran Miraç Yılmaz Naime Nur Bozbeyoğlu


This study was performed to determine the antimicrobial and antibiofilm activities of essential oil of Tanacetum argenteum (Lam.) Willd. subsp. canum (K.Koch) Grierson var. canum. The plant was collected from Amasya. The antimicrobial activity was determined by disc diffusion and microdilution broth methods. Essential oil was active against indicator organisms. The maximal inhibition zone diameter were as follows: E. cloacae ATCC 28355 (14mm), P. fluorescens ATCC 55241 (16mm), P. aeruginosa ATCC 27853 (19mm), S. sonnei RSKK 8177 (18mm), E. coli ATCC 25922 (21mm), E. coli O157:H7 (13mm), Y. enterocolitica RSKK 1501 (11mm), C. jejuni ATCC 33291 (12mm), K. pneumoniae ATCC 27736 (13mm), S. enteritidis RSKK 171 (12mm), S. aureus ATCC 33862 (26mm) and S. aureus ATCC 25923 (29mm),  M. luteus NRLL-B-4375 (20mm), E. faecalis ATCC 19433 (19mm), B. cereus NRRL-B-3711 (30mm), B. cereus RSKK (32mm), C. albicans ATCC 10231 (19mm) and C. tropicalis (17mm). The minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) were ranged from 62.5-900 µg/ml and 125-1000 µg/ml, respectively. Plant essential oil was the most active against B. cereus NRRL-B 3711 and B. subtilis RSKK 867. Also, the essential oil has shown a strong anti-candidal activity against two Candida species. For determining the antibiofilm effect, various dilutions of oil were studied on M. luteus, E. cloacae, P. fluorescens, Y. enterocolitica and S. enteritidis by using microplate biofilm assay. According to results, antibiofilm effect was found as weak. Maximum antibiofilm effect was determined on E. cloacae ATCC 28355 with 32.92% biofilm inhibition rate.

Article Details

Sending to International Journal of Secondary Metabolite
Author Biographies

Nazime Mercan Doğan, Pamukkale University


Arzu Cansaran, Amasya University

Department of Mathematics and Science Education Faculty of Education, Amasya University, Amasya, Turkey

Miraç Yılmaz, Hacettepe University

Department of Science and Mathematics Fields in Secondary Education, Faculty of Education, Hacettepe University, Ankara, Turkey

Naime Nur Bozbeyoğlu, Pamukkale University

Plant and Animal Production Department, Tavas Vocational High School, Pamukkale University, Denizli, Turkey


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