The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey

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Arzu KASKA

Abstract

Origanum genus is used as an anti-diabetic, diuretic, and for respiratory problems. Origanum sipyleum is a medicinal plant species belonging to the genus Origanum and traditionally vegetative parts of the plant have been used as medicinal tea or food additives .The current study has been designed to examine the antioxidant, cytotoxic activities and total phenolic, flavonoid and tannin content of the ethanol extract of O. sipyleum. The antioxidant capacity of  O. sipyleum was determined using  six complementary methods, DPPH free radical and ABTS radical cation scavenging assays, total antioxidant test systems (β-carotene/linoleic acid and phosphomolybdenum) , metal chelating and ferric ion reducing power assays. The phenolics were identified using HPLC. A brine shrimp (Artemia salina L.) lethality test was used for determining cytotoxic activity. The ethanol extract exhibited high DPPH free radical scavenging (DPPH, 102.75 µg/mL), ABTS radical scavenging (ABTS, 88.64 µg/mL), metal chelating (20.68 %) and ferric ion reducing power capacity (0.51 mg/mL). The antioxidant activities of the O. sipyleum with β-carotene /Linoleic acid and phosphomolybdenum were calculated as 85.59 % and 62.95 µg/mg respectively. The HPLC  results determined some phenolics: caffeic acid, epicatechin and 2,5 dihydroxybenzoic. Furthermore, to gether with cytotoxic activity (LC50, 327.414 µg/mL ). O. sipyleum is also rich in total phenolic, flavonoid and tannin content were 203.57 ± 4.62  mgGAE/g, 46.98 ± 0.34 mgQE/g and 34.55 ± 0.56 mgCE/g respectively. These results could provide addition information for the potential use of this medicinal plant as a food ingredient and as a natural antioxidant in the diet, as well as for the pharmaceutical industry.

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Sending to International Journal of Secondary Metabolite