Main Article Content
Skin is the largest organ of our body and it protects interior organs against several environmental factors. Hyperpigmentation problem occurs as a result of abnormal melanin accumulation in the skin. A considerable amount of world’s population uses skin whitening products. It is known that various algae derived secondary metabolites play an important role in skin problems. Therefore, the tyrosinase inhibitory activities of S. obliquus ethanol and water extracts were evaluated in the present study. Tyrosinase activity was determined spectrophotometrically at 492 nm. The ethanol extract showed the higher inhibitory activity on tyrosinase enzyme (IC50:0.0270g/mL) than water extract (IC50:0.2882g/mL). This result may be derived from the vanillic acid, ferulic acid and rutin components that were identified by RP-HPLC only in the ethanol extract.
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International Journal of Secondary Metabolite
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 Briganti, S., Camera, E., Picardo, M. (2003). Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell and Melanoma Research, 16, 101–110. Doi: 10.1034/j.1600-0749.2003.00029.x
 Mann, T., Gerwat, W., Batzer, J., Eggers, K., Scherner, C., Wenck, H., Stäb, F., Hearing, V.J., Röhm, K.H., Kolbe, L. (2018). Inhibition of Human Tyrosinase Requires Molecular Motifs Distinctively Different from Mushroom Tyrosinase. Journal of Investigative Dermatology, 138, 1601–1608. Doi: 10.1016/j.jid.2018.01.019
 Zolghadri, S., Bahrami, A., Khan, M.T.H., Munoz-Munoz, J., Garcia-Molina, F., Garcia-Canovas, F., Saboury, A.A. (2019). A comprehensive review on tyrosinase inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry, 34, 279–309. Doi: 10.1080/14756366.2018.1545767
 Scientiﬁc Committee on Consumer Products (2008), Opinion on b-arbutin. Retrieved at 21 November 2017 from: http://ec.europa.eu/health/archive/ph_risk/committees/04_sccp/docs/sccp_o_134.pdf
 Scientiﬁc Committee on Consumer Safety (2012), Opinion on kojic acid, Retrieved at 21 November 2017 from http://ec.europa.eu/health/scientiﬁc_committees/consumer_safety/docs/sccs_o_098.pdf
 Gao, H. (2018). Predicting tyrosinase inhibition by 3D QSAR pharmacophore models and designing potential tyrosinase inhibitors from Traditional Chinese medicine database. Phytomedicine, 38, 145–157. Doi: 10.1016/j.phymed.2017.11.012
 Cengiz Sahin, S. (2018). The potential of Arthrospira platensis extract as a tyrosinase inhibitor for pharmaceutical or cosmetic applications. South African Journal of Botany, 119, 236–243. Doi: 10.1016/j.sajb.2018.09.004
 Ariede, M.B., Candido, T.M., Jacome, A.L.M., Velasco, M.V.R., de Carvalho, J.C.M., Baby, A.R. (2017). Cosmetic attributes of algae - A review. Algal research, 25, 483–487. Doi: 10.1016/j.algal.2017.05.019
 Wang, H.D., Chen, C.C., Huynh, P., Chang, J.S. (2015). Exploring the potential of using algae in cosmetics Bioresource Technology, 184, 355 362. Doi: 10.1016/j.biortech.2014.12.001
 Park, Y.D., Lee, J.R., Park, K.H., Hahn, H.S., Hahn, M.J., Yang, J.M. (2003). A new continuous spectrophotometric assay method for DOPA oxidase activity of tyrosinase. Journal of Protein Chemistry, 22, 473–480. Doi: 10.1023/B:JOPC.0000005463.21302.cd
 Terpinc, P., Čeh, B., Ulrih, N.P., Abramovič, H. (2012). Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Industrial Crops and Products, 39, 210–217. Doi: 10.1016/j.indcrop.2012.02.023
 Caponio, F., Alloggio, V., Gomes, T. (1999). Phenolic compounds of virgin olive oil: inﬂuence of paste preperation techniques. Food Chemistry, 64, 203–209. Doi: 10.1016/S0308-8146(98)00146-0
 Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und Technologie, 28, 25–30. Doi: 10.1016/S0023-6438(95)80008-5
 Lee, S.Y., Baek, N., Nam, T.G. (2016). Natural, semisynthetic and synthetic tyrosinase inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(1), 1–13. Doi: 10.3109/14756366.2015.1004058
 Kang, H.S., Kim, H.R., Byun, D.S., Son, B.W., Nam, T.J., Choi, J.S. (2004). Tyrosinase inhibitors isolated from the edible brown alga Ecklonia stolonifera. Archives of Pharmacal Research, 27, 1226 - 1232. Doi: 10.1007/BF02975886Thomas, N.V., Kim, S.K. (2013). Beneﬁcial effects of marine algal compounds in cosmeceuticals. Marine Drugs, 11, 146–164. Doi: 10.3390/md11010146
 Yoon, N.Y., Eom, T.K., Kim, M.M., Kim, S.K. (2009). Inhibitory effect of phlorotannins isolated from Ecklonia cava on mushroom tyrosinase activity and melanin formation in mouse B16F10 melanoma cells. Journal of Agricultural and Food Chemistry, 57, 4124-4129. Doi: 10.1021/jf900006f
 Heo, S.J., Ko, S.C., Cha, S.H., Kang, D.H., Park, H.S., Choi, Y.U., Kim, D., Jung, W.K., Jeon, Y.J. (2009). Effect of phlorotannins isolated from Ecklonia cava on melanogenesis and their protective effect against photo-oxidative stress induced by UV-B radiation. Toxicology in Vitro, 23, 1123–1130. Doi: 10.1016/j.tiv.2009.05.013
 Loizzo, M.R., Tundis, R., Menichini, F. (2012). Natural and synthetic tyrosinase inhibitors as antibrowning agents: An update. Comprehensive Reviews in Food Science and Food Safety, 11, 378–398. Doi: 10.1111/j.1541-4337.2012.00191.x
 Pei, C.J., Lee, J., Si, Y.X., Oh, S., Xu, W.A., Yin, S.J., Qian, G.Y., Han, H.Y. (2013). Inhibition of tyrosinase by gastrodin: An integrated kinetic-computational simulation analysis. Process Biochemsitry, 48, 162–168. Doi: 10.1016/j.procbio.2012.11.004
 Chen, W.C., Tseng, T.S., Hsiao, N.W., Lin, Y.L., Wen, Z.H., Tsai, C.C., Lee, Y.C., Lin, H.H., Tsai, K.C. (2015). Discovery of highly potent tyrosinase inhibitor, T1, with Signiﬁcant anti-melanogenesis ability by zebraﬁsh in vivo assay and computational molecular modeling. Sci Rep, 5, 7995. Doi: 10.1038/srep07995
 Zheng, Z.P., Tan, H.Y., Chen, J., Wang, M. (2013). Characterization of tyrosinase inhibitors in th e twigs of Cudrania tricuspidata and their structure–activity relationship study. Fitoterapia, 84, 242–247. Doi: 10.1016/j.fitote.2012.12.006
 Stoica, R., Velea, S., Ilie, L., Calugareanu, M., Ghimis, S.B., Ion, R.M. (2013). The Influence of Ethanol Concentration on the Total Phenolics and Antioxidant Activity of Scenedesmus Opoliensis Algal Biomass Extracts. Revista de Chimie, 64, 304-306.
 Bulut, O., Akın, D., Sönmez, Ç., Öktem, A., Yücel, M., Öktem, H.A. (2019). Phenolic compounds, carotenoids, and antioxidant capacities of a thermo-tolerant Scenedesmus sp. (Chlorophyta) extracted with different solvents. Journal of Applied Phycology. Doi: 10.1007/s10811-018-1726-5
 Jerez-Martel, I., García-Poza, S., Rodríguez-Martel, G., Rico, M., Afonso-Olivares, C., Gómez-Pinchetti, J.L. (2017). Phenolic proﬁle and antioxidant activity of crude extracts from microalgae and cyanobacteria strains. Journal of Food Quality, 8 pages. Doi: 10.1155/2017/2924508
 Goiris, K., Muylaert, K., Fraeye, I., Foubert, I., De Brabanter, J., De Cooman, L. (2012) Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. Journal of Applied Phycology, 24, 1477–1486. Doi: 10.1007/s10811-012-9804-6
 Connan, S., Goulard, F., Stiger, V., Deslandes, E., Gall, E.A. (2004). Inter speciﬁc and temporal variation in phlorotannin levels in assemblage of brown algae. Botanica Marina, 47, 410–416. Doi: 10.1515/BOT.2004.057
 Marinho-Soriano, E., Fonseca, P.C., Carneiro, M.A., Moreira, W.S. (2006). Seasonal variation in the chemical composition of two tropical seaweeds. Bioresource Technology, 97, 2402–2406. Doi: 10.1016/j.biortech.2005.10.014
 Chou, T.H., Ding, H.Y., Hung, W.J., Liang, C.H. (2010). Antioxidative characteristics and inhibition of a-melanocyte-stimulating hormone-stimulated melanogenesis of vanillin and vanillic acid from Origanum vulgare. Experimental Dermatology, 19, 742–750. Doi: 10.1111/j.1600-0625.2010.01091.x
 Lee, H.S., Shin, K.H., Ryu, G.S., Chi, G.Y., Cho, I.S., Kim, H.Y. (2012). Synthesis of Small Molecule-Peptide Conjugates as Potential Whitening Agents. Bulletin Korean Chemical Society, 33, 3004–3008. Doi: 10.5012/bkcs.2012.33.9.3004
 Si, Y.X., Yin, S.J., Oh, S., Wang, Z.J., Ye, S., Yan, L., Yang, J.M., Park, Y.D., Lee, J., Qian, G.Y. (2012). An Integrated Study of Tyrosinase Inhibition by Rutin: Progress using a Computational Simulation. Journal of Biomolecular Structure and Dynamics, 29, 999–1012. Doi: 10.1080/073911012010525028
 Sabeena Farvin, K.H., Jacobsen, C. (2013). Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast. Food Chemistry, 138, 1670–1681. Doi: 10.1016/j.foodchem.2012.10.078