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The use of fruit and vegetable wastes in biogas production is attractive as it provides both waste treatment and energy production together. The use of CO2 in biogas for algae cultivation with a zero waste approach will make this process even more attractive. In this way, biogas enrichment which is mostly made by economically costly and non environmentally friendly methods will be achieved and algae cultivation will become more economical. In the first part of the study the operation conditions for biogas reactor and algea reactor for astaxhantin production were optimized separately. Up to 1.2 LCH4/day rate and 0.5 L CH4/g VS yield values were obtained with 2.5 g DM/L.day organic loading rate with anaerobic bioreactor and 5.1 mg/g astaxhantin was produced by air feeding. When it was decided that sufficient astaxanthin was produced, astaxanthin was obtained by using vegetable oils (oil and nut), an environmentally friendly extraction method. In the second part of the study the anaerobic bioreactor and the algae reactor was integrated and 6 mg/g astaxhantin production was observed by using fruit and vegetable wastes as substrate for biogas production and the CO2 in biogas was used for growth of H. pluvaris therefore astaxhantin production. The integrated system resulted in higher astaxhantin production with zero waste approach. Moreover the residual biomass remaining after extraction was fed back into the biogas reactor as a substrate, adopting a zero waste biorefinery approach.
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International Journal of Secondary Metabolite
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