The Comparison of Effects of Gamma Radiation of Crude Oil Yield on Some Sunflower (Helianthus annuus) Seeds

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This study compares the effects of different doses gamma radiation on crude oil yield and moisture of different six variety sunflower (Helianthus annuus L.) seeds. As materials, sunflower variety Ege-2001, Turay, AS-508, Tunca, TR-3080 and Tarsan-1018 seeds were used and irradiated with doses of 0 (control), 100, 200, 300, 400 and 500 Gy gamma radiation. Irradiation was performed in a cesium (Ce137) Gammacell 3000 Elan source, dose rate about 9.75 Gy/min (2900 Ci) in the Pamukkale University Faculty of Medicine in the department of the radiological. Moisture amount of seeds were also measured by AOCS standarts. Extraction of the seeds was done with soxhlet apparatus using petroleum ether by hot continuous extraction for 6 hours.  It was found that the highest moisture rate in 100 Gy for all seeds variety. The moisture rate ranged between 3.00 and 9.68% in TR-3080 and Ege-2001, respectively. According to the our results, seed moisture content was affected by gamma radiation in a significant negative one-way. The significant reduction in seed moisture content (9.68%) began at 100 Gy of gamma rays and continued to decline to up to 4.04% at 500 Gy. The crude oil yield showed not a important increase in 100 and 200 Gy doses. The result showed that the highest crude oil yield was also obtained from 400 Gy and 33.49% in Ege-2001 seeds.

Article Details

Sending to International Journal of Secondary Metabolite
Author Biographies

Havser ERTEM VAİZOĞULLAR, Pamukkale University


Yeşim KARA, Pamukkale University


Ayşe KURU, Pamukkale University



Ahmed, T.; Shittu, L.A.J.; Bankole, M.A.; Shittu, R.K.; Adesanya, O.A.; Bankole, M.N.; Ashiru, O.A. Comparative studies of the crude extracts of sesame against some common pathogenic microorganisms. Scientific Research and Essay. 2009, 4, 584-589.
AOAC. Official Methods of Analysis of the Association of Analtytical Chemists, (14Th ed., The William Byrd Press, Inc., Richmond, Virginia. 1984.
Bardakçı, B.; Seçilmiş, Canbay, H. Determination of Fatty Acid, C, H, N and Trace Element Composition in Grape Seed by GC/MS, FTIR, Elemental Analyzer and ICP/OES, SDU Journal of Science (E-Journal). 2011, 140-148.
Bhattacharya, S.; Joshi, R.K. Factors modifying radiation induced stimulation in plants: Pre-irradiation seed moisture content. Rad. Environ.Biophys. 1977, 14: 47-51.
Bewley, J.D. Seed germination and dormancy. Plant cell. 1997, 9 :1055-1066.
Brito, M.S.; Villavicencio, A.L.C.H.; Mancinifilho, J. Effects of irradiation on trans fatty acids formation in ground beef. Radiation Physics and Chemistry. 2002, 63 :337-340.
Collins, C.H. Microbiological Methods, (In: Lyne, P.M., Grange, J.M., Seventh ed. Butterworths, London. 1995.

Chaudhuri, S.K. A simple and reliable method to delect gamma irradiated lentil (Lens culinaris Medik.) seeds by germination efficiency and seedling growth test. Radiation Physics and Chemistry. 2002, 64: 131-136.
Cooney, R.V.; Custer, L.J.; Okinaka, L.; Franke, A.A. Effects of dietary sesame seeds on plasma tocopherol levels. Nutrition and Cancer. 2001, 39:66-71.
Das, A.; Data, S.; Mukherjee, S.; Bose, S.; Ghosh, S.; Dhar, P. Evaluation of antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and lignans. Food Science and Technology. 2015, 61: 244-250.
Elleuch, M.; Besbes, S.; Roiseux, O.; Blecker, C.; Attia, H. Quality characteristics of sesame seeds and by-products, Food Chemistry. 2007, 103: 641-650.
Elleuch, M.; Bedigian, D.; Zitoun, A. Sesame (Sesamum indicum L.) seeds in food, Nutrition and Health. Nuts & Seeds in Health and Disease Prevention. 2011, 122:1029-1036.
Gunckel, J.E.; Sparrow, A.H. Ionizing radiation: Biochemical, Physiological and Morphological aspects of their effects on plants. In: Encycl. Plant Physiol. Ed.Ruhland, W.XVI: Springer-verlag, Berlin. 1961.
Handel, A.P.; Nawar, W.W. Radiolytic Compounds from Mono-,Di- and Triacylglycerols. Radiat Res. 1981, 86: 428-436.
Jain, S.M.; Ahloowalia, B.S.; Veilleux, R.E. In:Somaclonal Variation and Induced Mutation in Crop Improvement (S.Mohan Jain, D.S. Brar, B.S. Ahloowalia, Eds.) Kluwer Academic Publishers, Great Britain. 1998.
Mahmoud, F.A.N. Effect of gamma radiation and some agrochemicals on germination, growth and flowering of Delphinium ajacis and Mathiola incana plants, M.Sc. Thesis, Moshtohor, Zagazig Univ., Fac. Agric, Egypt. 2002.
Nzikou, J.M.; Matos, L.; Bouanga-Kalou, G.; Ndangui, C.B.; Pambou-Tobi, N.P.G.; Kimbonguila, A.; Silou, T.; Linder, M.; Desobry, S. Chemical Composition on the Seeds and Oil of Sesame (Sesamum indicum L.) Grown in Congo-Brazzaville. Advance Journal of Food Science and Technology. 2009, 1: 6-11.
Ohba, K. Radiation sensitivity of pine seeds of different water content. Hereditas. 1961, 47: 283-294.
Özgen, M.; Turet, M.; Altinok, S.; Sancak, C. Effects callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L) genotypes. Plant Cell Rep. 1998, 18: 331-335.
Peters, J.; Lanham, B. Tetrazolium Testing handbook, Contribution no:29. Association of Official Seed Analysts, p.9-11. 1970.
Rahimi, M.M.; Bahrani, A. Effect of Gamma Irradiation on Qualitative and Quantitative Characteristics of Canola (Brassica napus L.), Middle-East Journal of Scientific Research. 2011, 8: 519-525.
Sabzalian, M.; Saeidi, G.; Mirlohi, A. Oil Content and Fatty Acid Composition in Seeds of Three Safflower Species. J Am Oil Chem Soc. 2008, 85: 717-721.
Sattar, A.; Ahmad, M.; Hussain, A.; Khan, I. Light induced oxidation of nut oils. Die Nahrung. 1989, 33: 213-215.
Saydut, A.; Du, M.Z.; Kaya, C.; Kafada, A.B.; Hamamci, C. Transesterified sesame (Sesamum indicum L.) seed oil as a biodiesel fuel. Bioresource Technology. 2008, 99: 6656-6660.
Selenia, L.V.; Stepanenko, O.G. Effect of presowing gamma irradiation on the productivity and active principle content of Matricaria recutita, Rastitel’nye Ressusy, 1979, 15: 143-154.
Uzun, B.; Arslan, C.; Karhan, M.; Toker, C. Fat and fatty acids of white lupin (Lupinus albus L.) in comparison to sesame (Sesamum indicum L.), Food Chemistry. 2007, 102: 45-49.
Victroria, A.; Corne, A.V.J.; Hamilton, J.T.G.; Stevenson, M.H. Detection of 2-dodecylcyclobutanone in radiation-sterilized chicken meat stored for several years. Int. J. Food Sci. Technol. 1992, 27: 691-696.
Weiss, E.A. Castor, sesame, and safltower. Barnes and Noble, Inc., New York. 1971.
Wi, S.G.; Chung, B.Y.; Kim, J.H.; Baek, M.H.; Yang, D.H.; Lee, J.W.; Kim, J.S. Ultra structural changes of cell organelles in Arabidopsis stem after gamma irradiation, J. Plant Biol. 2005, 48: 195-200.
Youssef, A.A.; Aly, M.S.; Hussein, M.S. Response of geranium (Pelargonium graveolenus L.) to gamma irradiation and foliar application of Speed Grow. Egyptian J. Hort. 2000, 27: 41-53.